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rnascope multiplex fluorescence in situ hybridization (fish)  (Advanced Cell Diagnostics Inc)


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    Advanced Cell Diagnostics Inc rnascope multiplex fluorescence in situ hybridization (fish)
    Localization of angiotensinogen (Agt) within the arcuate nucleus of the hypothalamus (ARC) and confirmation of cell-specific deletion of Agt in AgtLepr-KO and AgtAgrp-KO mice [mice exhibiting selective deletion of the Agt gene in cells that express either leptin receptor (Lepr) or agouti-related peptide (Agrp), respectively]. A: colocalization of Agt mRNA with mRNA for glial fibrillary acidic protein (Gfap), Lepr, proopiomelanocortin (Pomc), insulin II (Ins2), or Agrp in the ARC from a wild-type C57BL/6J mouse using in situ <t>hybridization/RNAscope.</t> DAPI, 4′,6-diamidino-2-phenylindole nuclear stain; ME, median eminence; 3V, third ventricle. B: loss of colocalization of Agt mRNA with Lepr in AgtLepr-KO mice. Black arrows identify a Lepr-positive (panel at left) but Agt-negative cell, whereas white arrows identify an Agt-positive, Gfap-positive neighboring cell. C: loss of colocalization of Agt mRNA with Agrp in AgtAgrp-KO mice. The black arrow identifies an Agrp-/Lepr-positive + Agt-negative cell, whereas the white arrow identifies an Agt-positive + Agrp-/Lepr-negative neighboring cell.
    Rnascope Multiplex Fluorescence In Situ Hybridization (Fish), supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnascope+multiplex+fluorescence+in+situ+hybridization+(fish)/rnascope+fluorescent+in+situ+hybridization/pmc07272763-97-13-24
    Average 90 stars, based on 1 article reviews
    rnascope multiplex fluorescence in situ hybridization (fish) - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Exploration of cardiometabolic and developmental significance of angiotensinogen expression by cells expressing the leptin receptor or agouti-related peptide"

    Article Title: Exploration of cardiometabolic and developmental significance of angiotensinogen expression by cells expressing the leptin receptor or agouti-related peptide

    Journal: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

    doi: 10.1152/ajpregu.00297.2019

    Localization of angiotensinogen (Agt) within the arcuate nucleus of the hypothalamus (ARC) and confirmation of cell-specific deletion of Agt in AgtLepr-KO and AgtAgrp-KO mice [mice exhibiting selective deletion of the Agt gene in cells that express either leptin receptor (Lepr) or agouti-related peptide (Agrp), respectively]. A: colocalization of Agt mRNA with mRNA for glial fibrillary acidic protein (Gfap), Lepr, proopiomelanocortin (Pomc), insulin II (Ins2), or Agrp in the ARC from a wild-type C57BL/6J mouse using in situ hybridization/RNAscope. DAPI, 4′,6-diamidino-2-phenylindole nuclear stain; ME, median eminence; 3V, third ventricle. B: loss of colocalization of Agt mRNA with Lepr in AgtLepr-KO mice. Black arrows identify a Lepr-positive (panel at left) but Agt-negative cell, whereas white arrows identify an Agt-positive, Gfap-positive neighboring cell. C: loss of colocalization of Agt mRNA with Agrp in AgtAgrp-KO mice. The black arrow identifies an Agrp-/Lepr-positive + Agt-negative cell, whereas the white arrow identifies an Agt-positive + Agrp-/Lepr-negative neighboring cell.
    Figure Legend Snippet: Localization of angiotensinogen (Agt) within the arcuate nucleus of the hypothalamus (ARC) and confirmation of cell-specific deletion of Agt in AgtLepr-KO and AgtAgrp-KO mice [mice exhibiting selective deletion of the Agt gene in cells that express either leptin receptor (Lepr) or agouti-related peptide (Agrp), respectively]. A: colocalization of Agt mRNA with mRNA for glial fibrillary acidic protein (Gfap), Lepr, proopiomelanocortin (Pomc), insulin II (Ins2), or Agrp in the ARC from a wild-type C57BL/6J mouse using in situ hybridization/RNAscope. DAPI, 4′,6-diamidino-2-phenylindole nuclear stain; ME, median eminence; 3V, third ventricle. B: loss of colocalization of Agt mRNA with Lepr in AgtLepr-KO mice. Black arrows identify a Lepr-positive (panel at left) but Agt-negative cell, whereas white arrows identify an Agt-positive, Gfap-positive neighboring cell. C: loss of colocalization of Agt mRNA with Agrp in AgtAgrp-KO mice. The black arrow identifies an Agrp-/Lepr-positive + Agt-negative cell, whereas the white arrow identifies an Agt-positive + Agrp-/Lepr-negative neighboring cell.

    Techniques Used: In Situ Hybridization, RNAscope, Staining

    Fluorescent in situ hybridization (RNAscope) detection of endogenous angiotensinogen (Agt) and agouti-related peptide (Agrp) mRNA in adrenal gland of a wild-type mouse. A: in situ hybridization to localize nuclei (DAPI, blue), Agt (green), and Agrp (red) in adrenal gland of a wild-type female mouse. B: image from inset of A, shown at greater magnification. C: similar in situ hybridization in adrenal gland of a wild-type male mouse. D: image from inset of C, shown at greater magnification.
    Figure Legend Snippet: Fluorescent in situ hybridization (RNAscope) detection of endogenous angiotensinogen (Agt) and agouti-related peptide (Agrp) mRNA in adrenal gland of a wild-type mouse. A: in situ hybridization to localize nuclei (DAPI, blue), Agt (green), and Agrp (red) in adrenal gland of a wild-type female mouse. B: image from inset of A, shown at greater magnification. C: similar in situ hybridization in adrenal gland of a wild-type male mouse. D: image from inset of C, shown at greater magnification.

    Techniques Used: In Situ Hybridization, RNAscope

    Related Articles

    Fluorescence In Situ Hybridization:

    Article Title: Coordinated differentiation of human intestinal organoids with functional enteric neurons and vasculature.
    Article Snippet: Rabbit anti-SM22 1:500 (Abcam Cat#ab14106), goat anti-E-Cadherin 1:500 (R&D Systems Cat# AF748), mouse anti-E-Cadherin 1:500 (BD Transduction Laboratories Cat# 610181), rabbit anti-PECAM 1:200 (Sigma Cat# HPA004690), mouse anti-TUBB3 1:200 (BioLegend Cat# 801201), sheep anti-SM22 1:500 (Novus Cat# AF7886), chicken anti-MAP2 1:2000 (Abcam Cat#ab92434), goat anti-VIM 1:200 (R&D Systems Cat#AF2105), rabbit anti-c-KIT 1:500 (AbcamCat#ab32363) andmouse anti-VE-CAD 1:1000 (R&D Systems Cat#MAB9381). .. FISH probes were acquired from ACDbio and stained using the RNAscope multiplex fluorescent manual protocol and kit. .. RNAscope Probe Hs-EREG (ACD, Cat# 313081).

    Article Title: Hypothalamic PNOC/NPY neurons constitute mediators of leptin-controlled energy homeostasis.
    Article Snippet: The tissue blocks was sectioned at 5 mm and mounted onto Fisher SuperFrost Plus glass slides (Fisher Scientific). .. Multiplex FISH was performed using the RNAscope LS multiplex fluorescent reagent kit and RNAscope LS 4-Plex Ancillary Kit Multiplex Reagent Kit (Advanced Cell Diagnostics, Bio-Techne, Cat #322800+#322830) together with iFluor488/iFluor546/ iFlour594 (1:500, AAT Bioquest/VWR, Cat #45020/45025/45035) and Opal690 Fluorophore Reagent pack detection (1:500, Cat #FP1497001KT, Akoya Biosciences) on a Leica BOND RX Fully Automated Research Stainer (Leica Biosystems) according to the manufacturer’s instructions. .. The human tissue sections were pre-treated for 30 min with HIER at 95 C in ER2 followed by 15 min protease treatment and then hybridized with human-specific probes to detect mRNA transcripts for AGRP (#557458), LEPR-TV1 (long isoform, #410378-C2), NPY (#416678-C3) and PNOC (#1045248-C4) (Advanced Cell Diagnostics, Bio-Techne).

    Staining:

    Article Title: Coordinated differentiation of human intestinal organoids with functional enteric neurons and vasculature.
    Article Snippet: Rabbit anti-SM22 1:500 (Abcam Cat#ab14106), goat anti-E-Cadherin 1:500 (R&D Systems Cat# AF748), mouse anti-E-Cadherin 1:500 (BD Transduction Laboratories Cat# 610181), rabbit anti-PECAM 1:200 (Sigma Cat# HPA004690), mouse anti-TUBB3 1:200 (BioLegend Cat# 801201), sheep anti-SM22 1:500 (Novus Cat# AF7886), chicken anti-MAP2 1:2000 (Abcam Cat#ab92434), goat anti-VIM 1:200 (R&D Systems Cat#AF2105), rabbit anti-c-KIT 1:500 (AbcamCat#ab32363) andmouse anti-VE-CAD 1:1000 (R&D Systems Cat#MAB9381). .. FISH probes were acquired from ACDbio and stained using the RNAscope multiplex fluorescent manual protocol and kit. .. RNAscope Probe Hs-EREG (ACD, Cat# 313081).

    RNAscope:

    Article Title: Coordinated differentiation of human intestinal organoids with functional enteric neurons and vasculature.
    Article Snippet: Rabbit anti-SM22 1:500 (Abcam Cat#ab14106), goat anti-E-Cadherin 1:500 (R&D Systems Cat# AF748), mouse anti-E-Cadherin 1:500 (BD Transduction Laboratories Cat# 610181), rabbit anti-PECAM 1:200 (Sigma Cat# HPA004690), mouse anti-TUBB3 1:200 (BioLegend Cat# 801201), sheep anti-SM22 1:500 (Novus Cat# AF7886), chicken anti-MAP2 1:2000 (Abcam Cat#ab92434), goat anti-VIM 1:200 (R&D Systems Cat#AF2105), rabbit anti-c-KIT 1:500 (AbcamCat#ab32363) andmouse anti-VE-CAD 1:1000 (R&D Systems Cat#MAB9381). .. FISH probes were acquired from ACDbio and stained using the RNAscope multiplex fluorescent manual protocol and kit. .. RNAscope Probe Hs-EREG (ACD, Cat# 313081).

    Article Title: Hypothalamic PNOC/NPY neurons constitute mediators of leptin-controlled energy homeostasis.
    Article Snippet: The tissue blocks was sectioned at 5 mm and mounted onto Fisher SuperFrost Plus glass slides (Fisher Scientific). .. Multiplex FISH was performed using the RNAscope LS multiplex fluorescent reagent kit and RNAscope LS 4-Plex Ancillary Kit Multiplex Reagent Kit (Advanced Cell Diagnostics, Bio-Techne, Cat #322800+#322830) together with iFluor488/iFluor546/ iFlour594 (1:500, AAT Bioquest/VWR, Cat #45020/45025/45035) and Opal690 Fluorophore Reagent pack detection (1:500, Cat #FP1497001KT, Akoya Biosciences) on a Leica BOND RX Fully Automated Research Stainer (Leica Biosystems) according to the manufacturer’s instructions. .. The human tissue sections were pre-treated for 30 min with HIER at 95 C in ER2 followed by 15 min protease treatment and then hybridized with human-specific probes to detect mRNA transcripts for AGRP (#557458), LEPR-TV1 (long isoform, #410378-C2), NPY (#416678-C3) and PNOC (#1045248-C4) (Advanced Cell Diagnostics, Bio-Techne).

    Article Title: Temporal refinement of Dach1 expression contributes to the development of somatosensory neurons.
    Article Snippet: washes and dehydration, the slides were mounted with Eukitt hardening mounting medium (Sigma). .. RNAScope multiplex fluorescent in situ hybridization (version 2) was carried out following the manufacturer’s protocol (Advanced Cell Diagnostics, ACD). .. The PCR product was then cloned into the TOPO II dual promoter vector (Promega) and the riboprobe synthesized (linearized with SpeI, transcribed with T7).

    Multiplex Assay:

    Article Title: Coordinated differentiation of human intestinal organoids with functional enteric neurons and vasculature.
    Article Snippet: Rabbit anti-SM22 1:500 (Abcam Cat#ab14106), goat anti-E-Cadherin 1:500 (R&D Systems Cat# AF748), mouse anti-E-Cadherin 1:500 (BD Transduction Laboratories Cat# 610181), rabbit anti-PECAM 1:200 (Sigma Cat# HPA004690), mouse anti-TUBB3 1:200 (BioLegend Cat# 801201), sheep anti-SM22 1:500 (Novus Cat# AF7886), chicken anti-MAP2 1:2000 (Abcam Cat#ab92434), goat anti-VIM 1:200 (R&D Systems Cat#AF2105), rabbit anti-c-KIT 1:500 (AbcamCat#ab32363) andmouse anti-VE-CAD 1:1000 (R&D Systems Cat#MAB9381). .. FISH probes were acquired from ACDbio and stained using the RNAscope multiplex fluorescent manual protocol and kit. .. RNAscope Probe Hs-EREG (ACD, Cat# 313081).

    Article Title: Hypothalamic PNOC/NPY neurons constitute mediators of leptin-controlled energy homeostasis.
    Article Snippet: The tissue blocks was sectioned at 5 mm and mounted onto Fisher SuperFrost Plus glass slides (Fisher Scientific). .. Multiplex FISH was performed using the RNAscope LS multiplex fluorescent reagent kit and RNAscope LS 4-Plex Ancillary Kit Multiplex Reagent Kit (Advanced Cell Diagnostics, Bio-Techne, Cat #322800+#322830) together with iFluor488/iFluor546/ iFlour594 (1:500, AAT Bioquest/VWR, Cat #45020/45025/45035) and Opal690 Fluorophore Reagent pack detection (1:500, Cat #FP1497001KT, Akoya Biosciences) on a Leica BOND RX Fully Automated Research Stainer (Leica Biosystems) according to the manufacturer’s instructions. .. The human tissue sections were pre-treated for 30 min with HIER at 95 C in ER2 followed by 15 min protease treatment and then hybridized with human-specific probes to detect mRNA transcripts for AGRP (#557458), LEPR-TV1 (long isoform, #410378-C2), NPY (#416678-C3) and PNOC (#1045248-C4) (Advanced Cell Diagnostics, Bio-Techne).

    Article Title: Temporal refinement of Dach1 expression contributes to the development of somatosensory neurons.
    Article Snippet: washes and dehydration, the slides were mounted with Eukitt hardening mounting medium (Sigma). .. RNAScope multiplex fluorescent in situ hybridization (version 2) was carried out following the manufacturer’s protocol (Advanced Cell Diagnostics, ACD). .. The PCR product was then cloned into the TOPO II dual promoter vector (Promega) and the riboprobe synthesized (linearized with SpeI, transcribed with T7).

    other:

    Article Title: Characterizing brainstem glucagon-like peptide-1 control of sensory-specific-satiety in male and female rats across the estrous cycle.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: Sertm2 is a conserved micropeptide that promotes GDNF-mediated motor neuron subtype specification.
    Article Snippet: SignalP-6.0 AlphaFold Jumper et al, 2021; Varadi et al, 2024 https:// alphafold.ebi.ac.uk/ smFISH and RNAScope Stellaris FISH Probes LGC Biosearch RNAscope Multiplex Fluorescent Reagent Kit v2 ACD Bio Cat. # 323100 RNAscope Probe- MmA730046J19Rik-O1 ACD Bio 1136231-C1 RNAscope Probe- Mm-Etv4-C2 ACD Bio 458121-C2 RNAscope Probe- Mm-Nr2f2-C2 ACD Bio 480301-C2 RNAscope Probe- Mm-Satb2-C3 ACD Bio 413261-C3 RNAscope Probe- Mm-Isl1-C3 ACD Bio 451931-C3 Opal 520 Reagent Pack ACD Bio FP1487001KT Opal 570 Reagent Pack ACD Bio FP1488001KT Opal 690 Reagent Pack ACD Bio FP1497001KT

    In Situ Hybridization:

    Article Title: Temporal refinement of Dach1 expression contributes to the development of somatosensory neurons.
    Article Snippet: washes and dehydration, the slides were mounted with Eukitt hardening mounting medium (Sigma). .. RNAScope multiplex fluorescent in situ hybridization (version 2) was carried out following the manufacturer’s protocol (Advanced Cell Diagnostics, ACD). .. The PCR product was then cloned into the TOPO II dual promoter vector (Promega) and the riboprobe synthesized (linearized with SpeI, transcribed with T7).



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    Image Search Results


    Localization of angiotensinogen (Agt) within the arcuate nucleus of the hypothalamus (ARC) and confirmation of cell-specific deletion of Agt in AgtLepr-KO and AgtAgrp-KO mice [mice exhibiting selective deletion of the Agt gene in cells that express either leptin receptor (Lepr) or agouti-related peptide (Agrp), respectively]. A: colocalization of Agt mRNA with mRNA for glial fibrillary acidic protein (Gfap), Lepr, proopiomelanocortin (Pomc), insulin II (Ins2), or Agrp in the ARC from a wild-type C57BL/6J mouse using in situ hybridization/RNAscope. DAPI, 4′,6-diamidino-2-phenylindole nuclear stain; ME, median eminence; 3V, third ventricle. B: loss of colocalization of Agt mRNA with Lepr in AgtLepr-KO mice. Black arrows identify a Lepr-positive (panel at left) but Agt-negative cell, whereas white arrows identify an Agt-positive, Gfap-positive neighboring cell. C: loss of colocalization of Agt mRNA with Agrp in AgtAgrp-KO mice. The black arrow identifies an Agrp-/Lepr-positive + Agt-negative cell, whereas the white arrow identifies an Agt-positive + Agrp-/Lepr-negative neighboring cell.

    Journal: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

    Article Title: Exploration of cardiometabolic and developmental significance of angiotensinogen expression by cells expressing the leptin receptor or agouti-related peptide

    doi: 10.1152/ajpregu.00297.2019

    Figure Lengend Snippet: Localization of angiotensinogen (Agt) within the arcuate nucleus of the hypothalamus (ARC) and confirmation of cell-specific deletion of Agt in AgtLepr-KO and AgtAgrp-KO mice [mice exhibiting selective deletion of the Agt gene in cells that express either leptin receptor (Lepr) or agouti-related peptide (Agrp), respectively]. A: colocalization of Agt mRNA with mRNA for glial fibrillary acidic protein (Gfap), Lepr, proopiomelanocortin (Pomc), insulin II (Ins2), or Agrp in the ARC from a wild-type C57BL/6J mouse using in situ hybridization/RNAscope. DAPI, 4′,6-diamidino-2-phenylindole nuclear stain; ME, median eminence; 3V, third ventricle. B: loss of colocalization of Agt mRNA with Lepr in AgtLepr-KO mice. Black arrows identify a Lepr-positive (panel at left) but Agt-negative cell, whereas white arrows identify an Agt-positive, Gfap-positive neighboring cell. C: loss of colocalization of Agt mRNA with Agrp in AgtAgrp-KO mice. The black arrow identifies an Agrp-/Lepr-positive + Agt-negative cell, whereas the white arrow identifies an Agt-positive + Agrp-/Lepr-negative neighboring cell.

    Article Snippet: Coronal sections of 10 μm were cut using a cryostat, and sections underwent RNAscope Multiplex fluorescence in situ hybridization (FISH) for fresh frozen samples (Advanced Cell Diagnostics).

    Techniques: In Situ Hybridization, RNAscope, Staining

    Fluorescent in situ hybridization (RNAscope) detection of endogenous angiotensinogen (Agt) and agouti-related peptide (Agrp) mRNA in adrenal gland of a wild-type mouse. A: in situ hybridization to localize nuclei (DAPI, blue), Agt (green), and Agrp (red) in adrenal gland of a wild-type female mouse. B: image from inset of A, shown at greater magnification. C: similar in situ hybridization in adrenal gland of a wild-type male mouse. D: image from inset of C, shown at greater magnification.

    Journal: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

    Article Title: Exploration of cardiometabolic and developmental significance of angiotensinogen expression by cells expressing the leptin receptor or agouti-related peptide

    doi: 10.1152/ajpregu.00297.2019

    Figure Lengend Snippet: Fluorescent in situ hybridization (RNAscope) detection of endogenous angiotensinogen (Agt) and agouti-related peptide (Agrp) mRNA in adrenal gland of a wild-type mouse. A: in situ hybridization to localize nuclei (DAPI, blue), Agt (green), and Agrp (red) in adrenal gland of a wild-type female mouse. B: image from inset of A, shown at greater magnification. C: similar in situ hybridization in adrenal gland of a wild-type male mouse. D: image from inset of C, shown at greater magnification.

    Article Snippet: Coronal sections of 10 μm were cut using a cryostat, and sections underwent RNAscope Multiplex fluorescence in situ hybridization (FISH) for fresh frozen samples (Advanced Cell Diagnostics).

    Techniques: In Situ Hybridization, RNAscope